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基于高效液相色谱-化学分类法的光合色素分析技术，结合多种水质综合评估方法，对2022年网湖10个测站进行了季度性采样和生态调查。结果表明：网湖水质介于Ⅱ类至Ⅴ类之间，主要特征光合色素为岩藻黄素(Fuco)、叶绿素b(Chl b)、叶绿素a(Chl a)、玉米黄素(Zea)和异黄素(Allo)。在不同季节中，网湖浮游植物群落结构的演替规律表现为：微型浮游植物的演替为裸藻+隐藻+硅藻→隐藻+裸藻+绿藻→裸藻→隐藻，超微型浮游植物的演替规律为裸藻+蓝藻+隐藻→裸藻+隐藻+蓝藻→裸藻→隐藻+绿藻。网湖四季的浮游植物群落变化与水体的主要理化因子(如水温、溶解氧、总氮、总磷等)密切相关。综合分析发现，网湖的富营养化程度在秋季达到中度，浮游植物的生物质量也在秋季达到峰值。研究揭示了水质、浮游植物群落变化及其影响因素，为网湖的生态环境监测和生物多样性保护提供了基础数据。
Abstract：Wanghu Lake, a freshwater ecosystem, was investigated to assess its water quality dynamics, phytoplankton community succession, and associated environmental drivers. It also conducted seasonal sampling and ecological surveys at 10 stations throughout the year of 2022. By employing high-performance liquid chromatography(HPLC) coupled with a chemical classification method, it analyzed photosynthetic pigments and integrated multiple comprehensive water quality indices for evaluation. The results demonstrated that the water quality of Wanghu Lake could be categorized between Class II and Class V. Dominant photosynthetic pigments, including fucoxanthin(Fuco), chlorophyll b(Chl b), chlorophyll a(Chl a), zeaxanthin(Zea), and alloxanthin(Allo), were identified as characteristic biomarkers. Seasonal succession patterns of phytoplankton communities were observed: for nanophytoplankton, the transition followed the sequence Euglenophytes + Cryptophytes + Diatoms → Cryptophytes + Euglenophytes + Chlorophytes → Euglenophytes → Cryptophytes, while picophytoplankton succession involved as Euglenophytes+ Cyanobacteria + Cryptophytes → Euglenophytes + Cryptophytes + Cyanobacteria → Euglenophytes → Cryptophytes + Chlorophytes. These shifts were closely correlated with key physicochemical factors, such as water temperature, dissolved oxygen, total nitrogen, and total phosphorus.Comprehensive analysis revealed that eutrophication in Wanghu Lake reached a moderate level during autumn, coinciding with the peak phytoplankton biomass.Focusing on the interplay between environmental parameters and biological communities, this study is hopeful to provide foundational data for ecological monitoring and biodiversity conservation strategies in Wanghu Lake.
[1]周道坤，刘晓伟，荣楠，等.湖北网湖自然保护区水质改善对策研究[J].环境科学与技术，2020,43(S2):255～261.
[2]沈贝贝，吴敬禄，曾海鳌，等.网湖沉积物正构烷烃分布特征及其记录的环境变化[J].环境科学，2017,38(9):3682～3688.
[3]Huisman J,Weissing F J.Biodiversity of plankton by species oscillations and chaos [J].Nature,1999,402(6760):407～410.
[4]Muhid P,Davis T W,Bunn S E,et al.Effects of inorganic nutrients in recycled water on freshwater phytoplankton biomass and composition [J].Water Research,2013,47(1):384～394.
[5]Nalewajko C,Murphy T P.Effects of temperature,and availability of nitrogen and phosphorus on the abundance of Anabaena and Microcystis in Lake Biwa,Japan:an experimental approach [J].Limnology,2001,2(1):45～48.
[6]王璐.氮、磷、硅、铁对小兴凯湖夏季浮游植物生长的影响[D].哈尔滨：东北林业大学，2015:1～52.
[7]Sláde■.Rotifers as indicators of water quality [J].Hydrobiologia,1983,100(1):169～201.
[8]李家园.基于 HPLC 光合色素分析技术对三峡水库超微型浮游植物生物多样性的研究[D].黄石：湖北师范学院，2014:19～21.
[9]Lu L,Jiang T,Xu Y,et al.Succession of phytoplankton functional groups from spring to early summer in the central Bohai Sea using HPLC-CHEMTAX approaches[J].Journal of Oceanography,2018,74(4):381～392.
[10]Madhu N V,Ullas N,Ashwini R,et al.Characterization of phytoplankton pigments and functional community structure in the Gulf of Mannar and the Palk Bay using HPLC-CHEMTAX analysis[J].Continental Shelf Research,2014,80:79～90.
[11]吴娅，王雨春，胡明明，等.三峡库区典型支流浮游细菌的生态分布及其影响因素[J].生态学杂志，2015,34(04):1060～1065.
[12]Conley D J,Paerl H W,Howarth R W,et al.2009.Ecology controlling eutrophication:nitrogen and phosphorus[J].Science,323:1014～1015.
[13]王旭，郭雯，王明果，等.人类活动影响下湖泊浮游植物碳氮稳定同位素变化特征—以异龙湖为例[J].中国环境科学，2023:1～14.
[14]Li L,Huang J Y,Dong X F.Nutrient release flux in Baiyangdian Lake with high disturbance[J].Chinese Journal of Analytical Chemistry,2022,50(8):100095.
[15]Melack JM,Kilham P,Fisher TR.Responses of phytoplankton to experimental fertilization with ammonium and phosphate in an African soda lake.Oecologia.1982 Jan;52(3):321～326.
[16]Klausmeier C A,Litchman E,Daufresne T,et al.Optimal nitrogen-to-phosphorus stoichiometry of phytoplankton [J].Nature,2004,429(6988):171～174.
[17]He S,Stevens R L S,Chan K L,et al.Ecophysiology of Freshwater Verrucomicrobia Inferred from Metagenome-Assembled Genomes[J].mSphere,2017,2(5):e00277～17.
[18]Qin B Q,Zhou J,Elser J J,et al.Water depth underpins the relative roles and fates of nitrogen and phosphorus in lakes[J].Environmental Science & Technology,2020,54(6):3191～3198.
[19]许海，秦伯强，朱广伟.太湖不同湖区夏季蓝藻生长的营养盐限制研究[J].中国环境科学，2012,32(12):2230～2236.
[20]Schindler D W,Hecky R E,Findlay D L,et al.Eutrophication of lakes cannot be controlled by reducing nitrogen input:results of a 37-year whole-ecosystem experiment[J].Proceedings of the National Academy of Sciences of the United States of America,2008,105(32):11254～11258.
[21]Ulrich S,Rita A,Lisette D S D,et al.Beyond the Plankton Ecology Group (PEG) Model:Mechanisms Driving Plankton Succession [J].Annual Review of Ecology,Evolution,and Systematics,2012,43(1):429～448.
[22]杨瑞丰.大连松树水库浮游植物特征及对环境因子的响应[J].中国水土保持，2019,(07):34～37.
[23]游亮，崔莉凤，刘载文，等.藻类生长过程中DO、pH与叶绿素相关性分析[J].环境科学与技术，2007,(09):42-44+117.
[24]赖俊翔，许铭本，庄军莲，等.广西北部湾近岸海域春季浮游植物群落结构特征及与环境因子的关系[J].海洋技术学报，2017,36(06):65～70.
[25]Jakobsen H H,Blanda E,Staehr A P,et al.Development of phytoplankton communities:Implications of nutrient injections on phytoplankton composition,pH and ecosystem production[J].Journal of Experimental Marine Biology and Ecology,2015,47381-89.
[26]王华，杨树平，房晟忠，等.滇池浮游植物群落特征及与环境因子的典范对应分析[J].中国环境科学，2016,36(02):544～552.
[27]孙丽丽.基于生态围隔的小型湖泊生态系统超微型真核浮游生物多样性研究[D].黄石：湖北师范大学，2022:1～73.
[28]葛秋诗，张萍，倪茂飞，等.典型喀斯特高原水库浮游植物与环境因子的关系[J].生态环境学报，2021,30(01):156～164.
[29]郑维发，曾昭琪.淡水蓝藻的高温适应[J].湖泊科学，1994,(4):356～363.
[30]谭梦婷，胡丽丽，杨春英，等.常德市城市水系及景观湖泊浮游植物群落结构及其对环境因子的响应[J].湖南文理学院学报(自然科学版),2022,34(03):70～75.
[31]吴转璋，朱超，唐萍，等.巢湖湖区浮游植物群落与水质因子相关性分析[J].生物学杂志，2023,40(01):79～84.
[32]邵星焱，金磊，张香港.生物特征与水污染关系的研究进展[J].农业与技术，2022,42(15):72～74.
[33]Jiang Y J,He W,Liu W X,et al.The seasonal and spatial variations of phytoplankton community and their correlation with environmental factors in a large eutrophic Chinese lake (Lake Chaohu) [J].Ecological Indicators,2014,40:58～67.
[34]ESTEVES-FERREIRA A A,INABA M,FORT A,et al.Nitrogen me-tabolism in cyanobacteria:metabolic and molecular control,growth consequences and biotechnological applications[J].Critical Reviews inMicrobiology,2018,44(5):541～560.
基本信息:
中图分类号:Q948.8;X173;X143
引用信息:
[1]侯建军,徐金晶,胡一晗,等.网湖微型浮游植物群落结构对水体营养状态变化的响应研究[J].湖北师范大学学报(自然科学版),2025,45(04):61-68.
基金信息:
国家自然科学基金(41171045)及学校配套项目; 湖北省中央引导地方科技发展专项(2017ZYYD008); 湖北名师工作室项目